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M94A0621.TXT
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1994-10-21
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Document 0621
DOCN M94A0621
TI Production of HIV DNA, mRNA and protein without infectious virus in
growth-arrested cells.
DT 9412
AU Kiernan R; Purcell D; McPhee D; National Centre in HIV Virology
Research, Cellular Biology Unit,; Macfarlane Burnet Centre for Medical
Research, Fairfield, Vic.
SO Annu Conf Australas Soc HIV Med. 1993 Oct 28-30;5:73 (abstract no. SB6).
Unique Identifier : AIDSLINE ASHM5/94349034
AB We have previously shown that an HTLV-I transformed cell line, MT-2,
arrested in cell division and proliferation by treatment with 5 mM
hydroxyurea could not be productively infected by HIV. Both release of
cell-free virus and cell-cell fusion were found to be inhibited.
Step-wise analyses of HIV replication have shown that complete reverse
transcripts are synthesized in growth arrested cells. Nuclear
translocation of viral DNA and subsequent production of both 1 and 2 LTR
circular forms were found to occur. However, integration was
significantly delayed. The expected range of spliced mRNAs was
detectable despite lack of viral integration. Furthermore, viral protein
could also be detected in the absence of integration. This data provides
further evidence for the role of unintegrated HIV DNA as a competent
template for transcription and translation of viral proteins. It also
suggests that integration of viral DNA is required for efficient virus
production since the presence of viral protein and RNA is insufficient
for particle formation. We are currently addressing the role of viral
and cellular factors in viral morphogenesis and cell-cell fusion in
growth arrested cells.
DE Cell Division/*GENETICS Cells, Cultured DNA, Viral/*GENETICS Gene
Expression Regulation, Viral/PHYSIOLOGY Human
HIV/*GENETICS/PATHOGENICITY HIV Antigens/*GENETICS Repetitive
Sequences, Nucleic Acid/GENETICS RNA Splicing/GENETICS RNA,
Messenger/*GENETICS Virulence/GENETICS Virus Replication/*GENETICS
MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).